Protein XRP2; RP2
|产品名称||Anti-RP2 Antibody(monoclonal, 3D7)|
|免疫原||E. coli-derived human RP2 recombinant protein (Position: D244-M348).|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||Protein XRP2 is a protein that in humans is encoded by the RP2 gene. It is mapped to Xp11.3. The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death. The RP2 protein is also involved in regulating the function and extension of outer segment of cone photoreceptors in mice.|
|研究类别||1. Chapple, J. P., Hardcastle, A. J., Grayson, C., Spackman, L. A., Willison, K. R., Cheetham, M. E.Mutations in the N-terminus of the X-linked retinitis pigmentosa protein RP2 interfere with the normal targeting of the protein to the plasma membrane. Hum. Molec. Genet. 9: 1919-1926, 2000.2. Evans, R. J., Schwarz, N., Nagel-Wolfrum, K., Wolfrum, U., Hardcastle, A. J., Cheetham, M. E.The retinitis pigmentosa protein RP2links pericentriolar vesicle transport between the Golgi and the primary cilium. Hum. Molec. Genet. 19: 1358-1367, 2010.|
|Uniprot ID||RP2: O75695|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Flow Cytometry||1-3μg/1x106 cells|
Figure 1. Western blot analysis of anti ZO-1 antibody (M01923).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Jurkat, whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human A549 whole cell lysates,Use rabbit anti- ZO-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.
Figure 2. ICC analysis of anti- RP2 antibody (M01923).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).
Figure 3.Flow cytometry analysis of 293T cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).