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Anti-RP2 Antibody(monoclonal, 3D7)

Protein XRP2; RP2

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一抗 - 单克隆抗体

说明书:

Figure 1. Western blot analysis of anti ZO-1 antibody (M01923).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Jurkat, whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human A549 whole cell lysates,Use rabbit anti- ZO-1  1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD. Figure 2. ICC analysis of anti- RP2 antibody (M01923).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue). Figure 3.Flow cytometry analysis of 293T cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
  • Figure 1. Western blot analysis of anti ZO-1 antibody (M01923).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Jurkat, whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human A549 whole cell lysates,Use rabbit anti- ZO-1  1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.
  • Figure 2. ICC analysis of anti- RP2 antibody (M01923).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).
  • Figure 3.Flow cytometry analysis of 293T cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

M01923

860元/50ul  1520元/100ul  2280元/150ul  

human, mouse, rat

WB,FCM

Monoclonal(Clone: 3D7)

现货

总价格:

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选择数量:

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Product Brief

  • 产品概况

    货号 M01923
    产品名称 Anti-RP2 Antibody(monoclonal, 3D7)
    基因名 RP2
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 3D7)
    抗体亚型 Mouse IgG2b
    分子量 40KD
    免疫原 E. coli-derived human RP2 recombinant protein (Position: D244-M348).
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 Protein XRP2 is a protein that in humans is encoded by the RP2 gene. It is mapped to Xp11.3. The RP2 locus has been implicated as one cause of X-linked retinitis pigmentosa. The predicted gene product shows homology with human cofactor C, a protein involved in the ultimate step of beta-tubulin folding. Progressive retinal degeneration may therefore be due to the accumulation of incorrectly folded photoreceptor or neuron-specific tubulin isoforms followed by progressive cell death. The RP2 protein is also involved in regulating the function and extension of outer segment of cone photoreceptors in mice.
    研究类别 1. Chapple, J. P., Hardcastle, A. J., Grayson, C., Spackman, L. A., Willison, K. R., Cheetham, M. E.Mutations in the N-terminus of the X-linked retinitis pigmentosa protein RP2 interfere with the normal targeting of the protein to the plasma membrane. Hum. Molec. Genet. 9: 1919-1926, 2000.2. Evans, R. J., Schwarz, N., Nagel-Wolfrum, K., Wolfrum, U., Hardcastle, A. J., Cheetham, M. E.The retinitis pigmentosa protein RP2links pericentriolar vesicle transport between the Golgi and the primary cilium. Hum. Molec. Genet. 19: 1358-1367, 2010.
    Uniprot ID RP2: O75695
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti ZO-1 antibody (M01923).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Jurkat, whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human A549 whole cell lysates,Use rabbit anti- ZO-1  1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.

    Figure 1. Western blot analysis of anti ZO-1 antibody (M01923).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Jurkat, whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human A549 whole cell lysates,Use rabbit anti- ZO-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for RP2 at approximately 40KD. The expected band size for RP2 is at 40KD.

    Figure 2. ICC analysis of anti- RP2 antibody (M01923).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).

    Figure 2. ICC analysis of anti- RP2 antibody (M01923).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).

    Figure 3.Flow cytometry analysis of 293T cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

    Figure 3.Flow cytometry analysis of 293T cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

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