1 Cys antibody|1 Cys peroxiredoxin antibody|1 Cys PRX antibody|1 cysPrx antibody|1-Cys peroxiredoxin antibody|1-Cys PRX antibody|24 kDa protein antibody|9430088D19Rik antibody|AA690119 antibody|Acidic calcium independent phospholipase A2 antibody|Acidic calcium-independent phospholipase A2 antibody|aiPLA2 antibody|Antioxidant protein 2 antibody|AOP2 antibody|Aop2 rs3 antibody|Brp 12 antibody|Ciliary body glutathione peroxidase antibody|CP 3 antibody|EC 188.8.131.52 antibody|EC 184.108.40.206 antibody|EC
|产品名称||Anti-PRDX6 Antibody(monoclonal, 6I8)|
|免疫原||E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6,respectively.|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||PRDX6 is also known as PRX,p29 or AOP2. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic,fatty acid,and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.|
|Uniprot ID||PRDX6: P30041|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Immunohistochemistry in paraffin section:||1:50-400|
|Immunocytochemistry in fixed cells:||1:50-400|
|Flow Cytometry:||1-3μg/1x106 cells|
|(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.|
Figure 1. Western blot analysis of anti- PRDX6 antibody (M01847-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela tissue lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human HEK293 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human SW620 whole cell lysates.Lane 6: rat RH35 whole cell lysates.Use rabbit anti- PRDX6 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for PRDX6 at approximately 29KD.The expected band size for PRDX6 is at 25KD.
Figure 2. ICC analysis of anti- PRDX6 antibody (M01847-1).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).
Figure 3.Flow cytometry analysis of Hela cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).