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ANTI-PCNA Antibody

Proliferating cell nuclear antigen; PCNA; Cyclin; PCNA

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一抗 - 内参抗体

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Figure 1. Western blot analysis of PCNA  using anti-PCNA  antibody (A00125). <br> The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Caco-2 whole cell lysates. <br>-PCNA  antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)  A specific band was detected for PCNA  at approximately 36KD. The expected band size for PCNA  is at 29KD. Figure 2. Western blot analysis of PCNA  using anti-PCNA  antibody (A00125). <br> The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat PC-12 whole cell lysates, <br>Lane 2: rat C6 whole cell lysates, <br>Lane 3: mouse NIH3T3 whole cell lysates, <br>Lane 4: mouse RAW246.7 whole cell lysates. <br>probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)  A specific band was detected for PCNA  at approximately 36KD. The expected band size for PCNA  is at 29KD. Figure 3. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of human intestinal cancer tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of human tonsil tissues.  Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of rat lymphaden tissues.  Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 6. Flow Cytometry analysis of 293T  cells using anti-PCNA antibody (A00125). <br>Overlay histogram showing 293T  cells stained with A00125 (Blue line). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
  • Figure 1. Western blot analysis of PCNA  using anti-PCNA  antibody (A00125). <br> The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Caco-2 whole cell lysates. <br>-PCNA  antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)  A specific band was detected for PCNA  at approximately 36KD. The expected band size for PCNA  is at 29KD.
  • Figure 2. Western blot analysis of PCNA  using anti-PCNA  antibody (A00125). <br> The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat PC-12 whole cell lysates, <br>Lane 2: rat C6 whole cell lysates, <br>Lane 3: mouse NIH3T3 whole cell lysates, <br>Lane 4: mouse RAW246.7 whole cell lysates. <br>probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)  A specific band was detected for PCNA  at approximately 36KD. The expected band size for PCNA  is at 29KD.
  • Figure 3. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of human intestinal cancer tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
  • Figure 4. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of human tonsil tissues.  Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
  • Figure 5. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of rat lymphaden tissues.  Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
  • Figure 6. Flow Cytometry analysis of 293T  cells using anti-PCNA antibody (A00125). <br>Overlay histogram showing 293T  cells stained with A00125 (Blue line). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

A00125

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB,IHC-P,FCM,Direct ELISA

Polyclonal

现货

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Product Brief

  • 产品概况

    货号 A00125
    产品名称 ANTI-PCNA Antibody
    基因名 PCNA
    抗体来源 Rabbit
    克隆 Polyclonal
    抗体亚型 Rabbit IgG
    分子量 29KD
    免疫原 E.coli-derived human PCNA recombinant protein (Position: M1-S261).
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 Immunogen affinity purified.
    浓度 Concentration: 0.1-0.5μg/ml; Tested Species: Human
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. It is mapped to 20p12.3. The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
    研究类别 1. Moldovan GL, Pfander B, Jentsch S (May 18, 2007). "PCNA, the maestro of the replication fork". Cell. 129 (4): 665–79. 2. Warbrick E (Mar 1998). "PCNA binding through a conserved motif". BioEssays. 20 (3): 195–9. 3. Mailand N, Gibbs-Seymour I, Bekker-Jensen S (May 2013). "Regulation of PCNA-protein interactions for genome stability". Nature Reviews. Molecular Cell Biology. 14 (5): 269–82.
    Uniprot ID PCNA: P12004
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。

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    Figure 1. Western blot analysis of PCNA  using anti-PCNA  antibody (A00125). <br> The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Caco-2 whole cell lysates. <br>-PCNA  antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)  A specific band was detected for PCNA  at approximately 36KD. The expected band size for PCNA  is at 29KD.

    Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (A00125).
    The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: human Caco-2 whole cell lysates.
    -PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.

    Figure 2. Western blot analysis of PCNA  using anti-PCNA  antibody (A00125). <br> The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat PC-12 whole cell lysates, <br>Lane 2: rat C6 whole cell lysates, <br>Lane 3: mouse NIH3T3 whole cell lysates, <br>Lane 4: mouse RAW246.7 whole cell lysates. <br>probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)  A specific band was detected for PCNA  at approximately 36KD. The expected band size for PCNA  is at 29KD.

    Figure 2. Western blot analysis of PCNA using anti-PCNA antibody (A00125).
    The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: rat PC-12 whole cell lysates,
    Lane 2: rat C6 whole cell lysates,
    Lane 3: mouse NIH3T3 whole cell lysates,
    Lane 4: mouse RAW246.7 whole cell lysates.
    probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for PCNA at approximately 36KD. The expected band size for PCNA is at 29KD.

    Figure 3. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of human intestinal cancer tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    Figure 3. IHC analysis of PCNA using anti-PCNA antibody (A00125).
    PCNA was detected in paraffin-embedded section of human intestinal cancer tissues.Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    Figure 4. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of human tonsil tissues.  Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    Figure 4. IHC analysis of PCNA using anti-PCNA antibody (A00125).
    PCNA was detected in paraffin-embedded section of human tonsil tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    Figure 5. IHC analysis of PCNA  using anti-PCNA  antibody (A00125). <br>PCNA  was detected in paraffin-embedded section of rat lymphaden tissues.  Biotinylated goat anti-rabbit IgG was used as secondary antibody  The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    Figure 5. IHC analysis of PCNA using anti-PCNA antibody (A00125).
    PCNA was detected in paraffin-embedded section of rat lymphaden tissues. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    Figure 6. Flow Cytometry analysis of 293T  cells using anti-PCNA antibody (A00125). <br>Overlay histogram showing 293T  cells stained with A00125 (Blue line). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

    Figure 6. Flow Cytometry analysis of 293T cells using anti-PCNA antibody (A00125).
    Overlay histogram showing 293T cells stained with A00125 (Blue line). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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