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Anti-MSI1 Antibody(monoclonal, 2B9)

Msi 1 | Msi1 | Musashi-1 | Musashi1 | O43347

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一抗 - 单克隆抗体

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Figure 1. Western blot analysis of anti- MSI antibody (M05052-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human PC-3 whole cell lysates,Use rabbit anti- MSI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for MSI at approximately 39KD. The expected band size for MSI is at 39KD. Figure 2. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of    human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of  human rectum cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. Figure 4.ICC analysis  using anti-MSI antibody (M05052-1) was detected in immersion fixed  MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog?#?BA1127) and counterstained with DAPI (blue).
  • Figure 1. Western blot analysis of anti- MSI antibody (M05052-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human PC-3 whole cell lysates,Use rabbit anti- MSI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for MSI at approximately 39KD. The expected band size for MSI is at 39KD.
  • Figure 2. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of    human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
  • Figure 3. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of  human rectum cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
  • Figure 4.ICC analysis  using anti-MSI antibody (M05052-1) was detected in immersion fixed  MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog?#?BA1127) and counterstained with DAPI (blue).

M05052-1

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB,IHC-P,ICC/IF

Monoclonal(Clone: 2B9)

现货

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Product Brief

  • 产品概况

    货号 M05052-1
    产品名称 Anti-MSI1 Antibody(monoclonal, 2B9)
    基因名 MSI1
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 2B9)
    抗体亚型 Mouse IgG2b
    分子量 39KD
    免疫原 A synthetic peptide corresponding to a sequence at the N-terminus of human Musashi 1/Msi1 (21-54aa KMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRD), identical to the related mouse and rat sequences.
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
    Uniprot ID MSI1: O43347
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti- MSI antibody (M05052-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human PC-3 whole cell lysates,Use rabbit anti- MSI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for MSI at approximately 39KD. The expected band size for MSI is at 39KD.

    Figure 1. Western blot analysis of anti- MSI antibody (M05052-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human PC-3 whole cell lysates,Use rabbit anti- MSI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for MSI at approximately 39KD. The expected band size for MSI is at 39KD.

    Figure 2. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of    human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 2. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 3. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of  human rectum cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 3. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of human rectum cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 4.ICC analysis  using anti-MSI antibody (M05052-1) was detected in immersion fixed  MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog?#?BA1127) and counterstained with DAPI (blue).

    Figure 4.ICC analysis using anti-MSI antibody (M05052-1) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog?#?BA1127) and counterstained with DAPI (blue).

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