Msi 1 | Msi1 | Musashi-1 | Musashi1 | O43347
|产品名称||Anti-MSI1 Antibody(monoclonal, 2B9)|
|免疫原||A synthetic peptide corresponding to a sequence at the N-terminus of human Musashi 1/Msi1 (21-54aa KMFIGGLSWQTTQEGLREYFGQFGEVKECLVMRD), identical to the related mouse and rat sequences.|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||RNA-binding protein Musashi homolog 1 is a protein that in humans is encoded by the MSI1 gene. This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.|
|Uniprot ID||MSI1: O43347|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Immunohistochemistry in paraffin section:||1:50-400|
|(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.|
Figure 1. Western blot analysis of anti- MSI antibody (M05052-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human PC-3 whole cell lysates,Use rabbit anti- MSI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for MSI at approximately 39KD. The expected band size for MSI is at 39KD.
Figure 2. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of anti- MSI antibody (M05052-1).detected in paraffin-embedded section of human rectum cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4.ICC analysis using anti-MSI antibody (M05052-1) was detected in immersion fixed MCF-7 cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog?#?BA1127) and counterstained with DAPI (blue).