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Anti-MPI Antibody(monoclonal, 11I4)

Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; PMI; MPI; PMI1

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一抗 - 单克隆抗体

说明书:

Figure 1. Western blot analysis of anti-MPI antibody (M00175).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysatesLane 2: human Hela whole cell lysatesUse rabbit anti- MPI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MPI at approximately 47KD. The expected band size for MPI is at 47KD. Figure 2.Flow cytometry analysis of SiHa cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
  • Figure 1. Western blot analysis of anti-MPI antibody (M00175).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysatesLane 2: human Hela whole cell lysatesUse rabbit anti- MPI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MPI at approximately 47KD. The expected band size for MPI is at 47KD.
  • Figure 2.Flow cytometry analysis of SiHa cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

M00175

860元/50ul  1520元/100ul  2280元/150ul  

human

WB,FCM

Monoclonal(Clone: 11I4)

现货

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Product Brief

  • 产品概况

    货号 M00175
    产品名称 Anti-MPI Antibody(monoclonal, 11I4)
    基因名 MPI
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 11I4)
    抗体亚型 Mouse IgG1
    分子量 47KD
    免疫原 E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate(F6P) and mannose-6-phosphate(M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
    研究类别 1. Jaeken, J., Matthijs, G., Saudubray, J.-M., Dionisi-Vici, C., Bertini, E., de Lonlay, P., Henri, H., Carchon, H., Schollen, E., Van Schaftingen, E. Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation. (Letter) Am. J. Hum. Genet. 62: 1535-1539, 1998.2. Schollen, E., Dorland, L., de Koning, T. J., Van Diggelen, O. P., Huijmans, J. G. M., Marquardt, T., Babovic-Vuksanovic, D., Patterson, M., Imtiaz, F., Winchester, B., Adamowicz, M., Pronicka, E., Freeze, H., Matthijs, G. Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib). Hum. Mutat. 16: 247-252, 2000.
    Uniprot ID MPI: P34949
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti-MPI antibody (M00175).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysatesLane 2: human Hela whole cell lysatesUse rabbit anti- MPI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MPI at approximately 47KD. The expected band size for MPI is at 47KD.

    Figure 1. Western blot analysis of anti-MPI antibody (M00175).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysatesLane 2: human Hela whole cell lysatesUse rabbit anti- MPI 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MPI at approximately 47KD. The expected band size for MPI is at 47KD.

    Figure 2.Flow cytometry analysis of SiHa cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

    Figure 2.Flow cytometry analysis of SiHa cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

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