Fzo homolog antibody|Hfzo1 antibody|Hfzo2 antibody|MFN 1 antibody|Mfn1 antibody|MFN1_HUMAN antibody|Mitochondrial transmembrane GTPase Fzo 1 antibody|Mitochondrial transmembrane GTPase FZO 2 antibody|Mitochondrial transmembrane GTPaseFZO1B antibody|Mitofusin 1 antibody|Mitofusin-1 antibody|Mitofusin1 antibody|Putative transmembrane GTPase antibody|Transmembrane GTPase MFN1 antibody
|产品名称||Anti-MFN1 Antibody(monoclonal, 3H3)|
|免疫原||A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1 (23-49aa DQLLEFVTEGSHFVEATYKNPELDRIA),different from the related mouse and rat sequences by one amino acid.|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80 90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed,and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion,and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers,either with itself or MFN 2,and to undergo ubiquitination by MARCH5. MFN 1 has two key domains. One is a coiled°Coil region that mediates MFN 1: MFN 1/2 binding,and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.|
|Uniprot ID||MFN1: Q8IWA4|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Immunocytochemistry in fixed cells:||1:50-400|
Figure 1. Western blot analysis of anti- MFN1 antibody (M02172-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela tissue lysates,Lane 2: human HepG2 whole cell lysates,Use rabbit anti- MFN1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for MFN1 at approximately 84KD. The expected band size for MFN1 is at 84KD.
Figure 2. ICC analysis of anti- MFN1 antibody (M02172-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).