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Anti-IDH1 Antibody(monoclonal, 16H7)

Cytosolic NADP isocitrate dehydrogenase antibody|Cytosolic NADP-isocitrate dehydrogenase antibody|Epididymis luminal protein 216 antibody|Epididymis secretory protein Li 26 antibody|HEL-216 antibody|HEL-S-26 antibody|ICDH antibody|IDCD antibody|IDH antibody|IDH1 antibody|IDHC_HUMAN antibody|IDP antibody|IDPC antibody|Isocitrate dehydrogenase [NADP] cytoplasmic antibody|Isocitrate dehydrogenase 1 (NADP+) soluble antibody|NADP dependent isocitrate dehydrogenase cytosolic antibody|NADP dependent is

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一抗 - 单克隆抗体

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Figure 1. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: human THP-1 whole cell lysates,Lane 5: human Hela whole cell lysates.Lane 6: human K562 whole cell lysates.Lane 7: human PC-3 whole cell lysates.Lane 8: human HEK293 whole cell lysates.Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD. Figure 2. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat RH35 whole cell lysates,Lane 3: mouse liver whole cell lysates,Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD. Figure 3. ICC analysis of anti- IDH1 antibody (M00129-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).
  • Figure 1. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: human THP-1 whole cell lysates,Lane 5: human Hela whole cell lysates.Lane 6: human K562 whole cell lysates.Lane 7: human PC-3 whole cell lysates.Lane 8: human HEK293 whole cell lysates.Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.
  • Figure 2. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat RH35 whole cell lysates,Lane 3: mouse liver whole cell lysates,Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.
  • Figure 3. ICC analysis of anti- IDH1 antibody (M00129-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).

M00129-1

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB,ICC/IF,FCM

Monoclonal(Clone: 16H7)

现货

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Product Brief

  • 产品概况

    货号 M00129-1
    产品名称 Anti-IDH1 Antibody(monoclonal, 16H7)
    基因名 IDH1
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 16H7)
    抗体亚型 Mouse IgG1
    分子量 47KD
    免疫原 A synthetic peptide corresponding to a sequence at the C-terminus of human IDH1 (381-413aa KGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAK),different from the related mouse and rat sequences by one amino acid.
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 Isocitrate dehydrogenase 1 (NADP+),soluble is an enzyme that in humans is encoded by the IDH1 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses,one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases,which localize to the mitochondrial matrix,and two NADP(+)-dependent isocitrate dehydrogenases,one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions,such as the conversion of 2,4-dienoyl-CoAs to 3-enoyl-CoAs,as well as in peroxisomal reactions that consume 2-oxoglutarate,namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
    Uniprot ID IDH1: O75874
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: human THP-1 whole cell lysates,Lane 5: human Hela whole cell lysates.Lane 6: human K562 whole cell lysates.Lane 7: human PC-3 whole cell lysates.Lane 8: human HEK293 whole cell lysates.Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.

    Figure 1. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: human THP-1 whole cell lysates,Lane 5: human Hela whole cell lysates.Lane 6: human K562 whole cell lysates.Lane 7: human PC-3 whole cell lysates.Lane 8: human HEK293 whole cell lysates.Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.

    Figure 2. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat RH35 whole cell lysates,Lane 3: mouse liver whole cell lysates,Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.

    Figure 2. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat RH35 whole cell lysates,Lane 3: mouse liver whole cell lysates,Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.

    Figure 3. ICC analysis of anti- IDH1 antibody (M00129-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).

    Figure 3. ICC analysis of anti- IDH1 antibody (M00129-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).

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