Cytosolic NADP isocitrate dehydrogenase antibody|Cytosolic NADP-isocitrate dehydrogenase antibody|Epididymis luminal protein 216 antibody|Epididymis secretory protein Li 26 antibody|HEL-216 antibody|HEL-S-26 antibody|ICDH antibody|IDCD antibody|IDH antibody|IDH1 antibody|IDHC_HUMAN antibody|IDP antibody|IDPC antibody|Isocitrate dehydrogenase [NADP] cytoplasmic antibody|Isocitrate dehydrogenase 1 (NADP+) soluble antibody|NADP dependent isocitrate dehydrogenase cytosolic antibody|NADP dependent is
|产品名称||Anti-IDH1 Antibody(monoclonal, 16H7)|
|免疫原||A synthetic peptide corresponding to a sequence at the C-terminus of human IDH1 (381-413aa KGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAK),different from the related mouse and rat sequences by one amino acid.|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||Isocitrate dehydrogenase 1 (NADP+),soluble is an enzyme that in humans is encoded by the IDH1 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses,one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases,which localize to the mitochondrial matrix,and two NADP(+)-dependent isocitrate dehydrogenases,one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions,such as the conversion of 2,4-dienoyl-CoAs to 3-enoyl-CoAs,as well as in peroxisomal reactions that consume 2-oxoglutarate,namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.|
|Uniprot ID||IDH1: O75874|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Immunocytochemistry in fixed cells:||1:50-400|
|Flow Cytometry:||1-3μg/1x106 cells|
Figure 1. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: human THP-1 whole cell lysates,Lane 5: human Hela whole cell lysates.Lane 6: human K562 whole cell lysates.Lane 7: human PC-3 whole cell lysates.Lane 8: human HEK293 whole cell lysates.Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.
Figure 2. Western blot analysis of anti- IDH1 antibody (M00129-1).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: rat RH35 whole cell lysates,Lane 3: mouse liver whole cell lysates,Use rabbit anti- IDH1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for IDH1 at approximately 47KD. The expected band size for IDH1 is at 47KD.
Figure 3. ICC analysis of anti- IDH1 antibody (M00129-1).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127)and counterstained with DAPI (blue).