|产品名称||Anti-HOMER3 Antibody (monoclonal, 9G13)|
|免疫原||E. coli-derived human HOMER3 recombinant protein (Position: R282-A360). Human HOMER3 shares 88.6% and 89.9% amino acid (aa) sequence identity with mouse and rat HOMER3, respectively.|
|内容||200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|Uniprot ID||HOMER3: Q9NSC5|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。|
|Flow Cytometry:||1-3μg/1x106 cells|
Figure 1. Western blot analysis of anti-HOMER3 antibody (M09145). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human U-87MG tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Use rabbit anti- HOMER3 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for HOMER3 at approximately 45KD. The expected band size for HOMER3 is at 40KD.
Figure 2.Flow cytometry analysis of U937 cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).