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Anti-HMGB3 Antibody(monoclonal, 8H9)

chromosomal protein,Nonhistone,HMG4 antibody|High mobility group (nonhistone chromosomal) protein 4 antibody|High mobility group box 3 antibody|High mobility group protein 2a antibody|High mobility group protein 4 antibody|High mobility group protein B3 antibody|High mobility group protein HMG4 antibody|HMG 4 antibody|HMG-2a antibody|HMG-4 antibody|HMG2A antibody|HMGB 3 antibody|HMGB3 antibody| HMGB3_HUMAN antibody|MGC90319 antibody|Non histone chromosomal protein antibody|Nonhistone chromosoma

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一抗 - 单克隆抗体

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Figure 1. Western blot analysis of anti- HMGB3 antibody (M02834).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human T-47D whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human Caco-2 whole cell lysates.Lane 7: human SW620 whole cell lysates.Lane 8: human Raji whole cell lysates.Use rabbit anti- HMGB3 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for HMGB3 at approximately 23KD. The expected band size for HMGB3 is at 23KD. Figure 2. IHC analysis of anti- HMGB3 antibody (M02834).detected in paraffin-embedded section of    human placenta cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. Figure 3. ICC analysis of anti-  HMGB3 antibody (M02834).was detected in immunocytochemical section of Hela cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue). Figure 4.Flow cytometry analysis of Hela cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
  • Figure 1. Western blot analysis of anti- HMGB3 antibody (M02834).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human T-47D whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human Caco-2 whole cell lysates.Lane 7: human SW620 whole cell lysates.Lane 8: human Raji whole cell lysates.Use rabbit anti- HMGB3 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for HMGB3 at approximately 23KD. The expected band size for HMGB3 is at 23KD.
  • Figure 2. IHC analysis of anti- HMGB3 antibody (M02834).detected in paraffin-embedded section of    human placenta cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
  • Figure 3. ICC analysis of anti-  HMGB3 antibody (M02834).was detected in immunocytochemical section of Hela cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue).
  • Figure 4.Flow cytometry analysis of Hela cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

M02834

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB,IHC-P,ICC/IF,FCM

Monoclonal(Clone: 8H9)

现货

总价格:

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Product Brief

  • 产品概况

    货号 M02834
    产品名称 Anti-HMGB3 Antibody(monoclonal, 8H9)
    基因名 HMGB3
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 8H9)
    抗体亚型 Mouse IgG2b
    分子量 23KD
    免疫原 A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4 (62-95aa EMAKADKVRYDREMKDYGPAKGGKKKKDPNAPKR),identical to the related mouse and rat sequences.
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 High-mobility group protein B,also known as HMG4,is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations,and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia,syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.
    Uniprot ID HMGB3: O15347
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti- HMGB3 antibody (M02834).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human T-47D whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human Caco-2 whole cell lysates.Lane 7: human SW620 whole cell lysates.Lane 8: human Raji whole cell lysates.Use rabbit anti- HMGB3 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for HMGB3 at approximately 23KD. The expected band size for HMGB3 is at 23KD.

    Figure 1. Western blot analysis of anti- HMGB3 antibody (M02834).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human Hela whole cell lysates,Lane 3: human T-47D whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human Caco-2 whole cell lysates.Lane 7: human SW620 whole cell lysates.Lane 8: human Raji whole cell lysates.Use rabbit anti- HMGB3 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for HMGB3 at approximately 23KD. The expected band size for HMGB3 is at 23KD.

    Figure 2. IHC analysis of anti- HMGB3 antibody (M02834).detected in paraffin-embedded section of    human placenta cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 2. IHC analysis of anti- HMGB3 antibody (M02834).detected in paraffin-embedded section of human placenta cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 3. ICC analysis of anti-  HMGB3 antibody (M02834).was detected in immunocytochemical section of Hela cells. Cells were stained using the Dylight488-conjugated Anti-rabbit  IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue).

    Figure 3. ICC analysis of anti- HMGB3 antibody (M02834).was detected in immunocytochemical section of Hela cells. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue).

    Figure 4.Flow cytometry analysis of Hela cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

    Figure 4.Flow cytometry analysis of Hela cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

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