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Anti-GAA Antibody(monoclonal, 2G7)

Acid alpha glucosidase | Acid maltase | Aglucosidase alfa | Alpha glucosidase | GAA | Glucosidase alpha acid | Glucosidase alpha | LYAG | P10253

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一抗 - 单克隆抗体

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Figure 1. Western blot analysis of anti- GAA antibody (M01548).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Use rabbit anti- GAA 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD. Figure 2. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of   human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of   human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of    human prostatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. Figure 5. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit  IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue). Figure 6. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit  IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue). Figure 7.Flow cytometry analysis of THP-1 cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
  • Figure 1. Western blot analysis of anti- GAA antibody (M01548).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Use rabbit anti- GAA 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.
  • Figure 2. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of   human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
  • Figure 3. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of   human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
  • Figure 4. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of    human prostatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
  • Figure 5. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit  IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).
  • Figure 6. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit  IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).
  • Figure 7.Flow cytometry analysis of THP-1 cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

M01548

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB,IHC-P,ICC/IF,FCM

Monoclonal(Clone: 2G7)

现货

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Product Brief

  • 产品概况

    货号 M01548
    产品名称 Anti-GAA Antibody(monoclonal, 2G7)
    基因名 GAA
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 2G7)
    抗体亚型 Mouse IgG2b
    分子量 110/95/76KD
    免疫原 A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin(1-26aa MLLLADMDVVNQLVAGGQFRVVKEPL),identical to the related mouse and rat sequences.
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 Lysosomal alpha-glucosidase is an enzyme that in humans is encoded by the GAA gene. This gene encodes lysosomal alpha-glucosidase,which is essential for the degradation of glycogen to glucose in lysosomes. The encoded preproprotein is proteolytically processed to generate multiple intermediate forms and the mature form of the enzyme. Defects in this gene are the cause of glycogen storage disease II,also known as Pompe's disease,which is an autosomal recessive disorder with a broad clinical spectrum. Alternative splicing results in multiple transcript variants.
    Uniprot ID GAA: P10253
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti- GAA antibody (M01548).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Use rabbit anti- GAA 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.

    Figure 1. Western blot analysis of anti- GAA antibody (M01548).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 tissue lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Use rabbit anti- GAA 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002).A specific band was detected for GAA at approximately 110,95,76KD. The expected band size for GAA is at 105KD.

    Figure 2. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of   human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 2. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 3. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of   human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 3. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 4. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of    human prostatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 4. IHC analysis of anti- GAA antibody (M01548).detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 5. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit  IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).

    Figure 5. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).

    Figure 6. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit  IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).

    Figure 6. ICC analysis of anti- GAA antibody (M01548).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)Catalog # BA1032)and counterstained with DAPI (blue).

    Figure 7.Flow cytometry analysis of THP-1 cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

    Figure 7.Flow cytometry analysis of THP-1 cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

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