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Anti-CD5 Antibody(monoclonal, 4E2)

T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD5; CD5; LEU1

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一抗 - 单克隆抗体

说明书:

Figure 1. Western blot analysis of anti- CD5 antibody (M00480-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat tissue lysates,Lane 2: human CCRM-CEM whole cell lysates,Use rabbit anti- CD5 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD. Figure 2. IHC analysis of anti- CD5 antibody (M00480-2).detected in paraffin-embedded section of  human tonsil cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
  • Figure 1. Western blot analysis of anti- CD5 antibody (M00480-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat tissue lysates,Lane 2: human CCRM-CEM whole cell lysates,Use rabbit anti- CD5 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.
  • Figure 2. IHC analysis of anti- CD5 antibody (M00480-2).detected in paraffin-embedded section of  human tonsil cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

M00480-2

860元/50ul  1520元/100ul  2280元/150ul  

human

WB,IHC-P

Monoclonal(Clone: 4E2)

现货

总价格:

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选择数量:

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Product Brief

  • 产品概况

    货号 M00480-2
    产品名称 Anti-CD5 Antibody(monoclonal, 4E2)
    基因名 CD5
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 4E2)
    抗体亚型 Mouse IgG1
    分子量 67KD
    免疫原 E. coli-derived human CD5 recombinant protein (Position: R25-L495).
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.
    研究类别 1. Lundell, A.-C., Johansen, S., Adlerberth, I., Wold, A. E., Hesselmar, B., Rudin, A. High proportion of CD5+ B cells in infants predicts development of allergic disease. J. Immun. 193: 510-518, 2014.2. Zhang, C., Xin, H., Zhang, W., Yazaki, P. J., Zhang, Z., Le, K., Li, W., Lee, H., Kwak, L., Forman, S., Jove, R., Yu, H. CD5 binds to interleukin-6 and induces a feed-forward loop with the transcription factor STAT3 in B cells to promote cancer. Immunity 44: 913-923, 2016.
    Uniprot ID CD5: P06127
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti- CD5 antibody (M00480-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat tissue lysates,Lane 2: human CCRM-CEM whole cell lysates,Use rabbit anti- CD5 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.

    Figure 1. Western blot analysis of anti- CD5 antibody (M00480-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Jurkat tissue lysates,Lane 2: human CCRM-CEM whole cell lysates,Use rabbit anti- CD5 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD.

    Figure 2. IHC analysis of anti- CD5 antibody (M00480-2).detected in paraffin-embedded section of  human tonsil cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    Figure 2. IHC analysis of anti- CD5 antibody (M00480-2).detected in paraffin-embedded section of human tonsil cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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