AHUS2 antibody|Antigen defined by monoclonal antibody| TRA 2 10 antibody|Antigen identified by monoclonal antibody| TRA 2 10 antibody|CD46 antibody|CD46 antigen antibody|CD46 antigen complement regulatory protein antibody|CD46 molecule antibody|CD46 molecule complement regulatory protein antibody|Complement membrane cofactor protein antibody|MCP antibody| MCP_HUMAN antibody|Measles virus receptor antibody|membrane cofactor protein (CD46,trophoblast-lymphocyte cross-reactive antigen) antibody|Mem
|产品名称||Anti-CD46 Antibody(monoclonal, 9E9)|
|免疫原||A synthetic peptide corresponding to a sequence at the C-terminus of human CD46 (366-392aa YRYLQRRKKKGTYLTDETHREVKFTSL).|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||CD46 complement regulatory protein also known as CD46 (cluster of differentiation 46) and Membrane Cofactor Protein is a protein which in humans is encoded by the CD46 gene. The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. And the encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I,which protects the host cell from damage by complement. In addition,the encoded protein can act as a receptor for the Edmonston strain of measles virus,human herpesvirus-6,and type IV pili of pathogenic Neisseria. Finally,the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.|
|Uniprot ID||CD46: P15529|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Immunohistochemistry in paraffin section:||1:50-400|
|Flow Cytometry:||1-3μg/1x106 cells|
|(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.|
Figure 1. Western blot analysis of anti ZO-1 antibody (M00377-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HepG2 tissue lysates,Use rabbit anti- ZO-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD46 at approximately 50-70KD. The expected band size for CD46 is at 44KD.
Figure 2. IHC analysis of anti- CD46 antibody (M00377-2).detected in paraffin-embedded section of human rectum cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of anti- CD46 antibody (M00377-2).detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4.Flow cytometry analysis of PBMC cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).