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Anti-CD22 Antibody(monoclonal, 5E7)

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD22; SIGLEC2

分类:

一抗 - 单克隆抗体

说明书:

Figure 1. Western blot analysis of anti ZO-1 antibody (M01572-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Raji tissue lysates,Use rabbit anti- ZO-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD22 at approximately 140KD. The expected band size for CD22is at 95KD. Figure 2.Flow cytometry analysis of PBMC cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
  • Figure 1. Western blot analysis of anti ZO-1 antibody (M01572-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Raji tissue lysates,Use rabbit anti- ZO-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD22 at approximately 140KD. The expected band size for CD22is at 95KD.
  • Figure 2.Flow cytometry analysis of PBMC cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

M01572-2

860元/50ul  1520元/100ul  2280元/150ul  

human

WB,FCM

Monoclonal(Clone: 5E7)

现货

总价格:

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选择数量:

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Product Brief

  • 产品概况

    货号 M01572-2
    产品名称 Anti-CD22 Antibody(monoclonal, 5E7)
    基因名 CD22
    抗体来源 Mouse
    克隆 Monoclonal(Clone: 5E7)
    抗体亚型 Mouse IgG2b
    分子量 140KD
    免疫原 A synthetic peptide corresponding to a sequence at the C-terminus of human CD22 (696-724aa LAILILAICGLKLQRRWKRTQSQQGLQEN), different from the related mouse sequence by ten amino acids.
    内容 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
    纯化方式 protein G purified.
    浓度 500ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    背景资料 CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.
    研究类别 1. John, B.; Herrin, B. R.; Raman, C.; Wang, Y.; Bobbitt, K. R.; Brody, B. A.; Justement, L. B. The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit adapter protein, via tyrosine-dependent interaction. J. Immun. 170: 3534-3543, 2003. 2. O'Keefe, T. L.; Williams, G. T.; Davies, S. L.; Neuberger, M. S. Hyperresponsive B cells in CD22-deficient mice. Science 274: 798-801, 1996.3. Wilson, G. L.; Najfeld, V.; Kozlow, E.; Menniger, J.; Ward, D.; Kehrl, J. H. Genomic structure and chromosomal mapping of the human CD22 gene. J. Immun. 150: 5013-5024, 1993.
    Uniprot ID CD22: P20273
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

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    Figure 1. Western blot analysis of anti ZO-1 antibody (M01572-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Raji tissue lysates,Use rabbit anti- ZO-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD22 at approximately 140KD. The expected band size for CD22is at 95KD.

    Figure 1. Western blot analysis of anti ZO-1 antibody (M01572-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Raji tissue lysates,Use rabbit anti- ZO-1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CD22 at approximately 140KD. The expected band size for CD22is at 95KD.

    Figure 2.Flow cytometry analysis of PBMC cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

    Figure 2.Flow cytometry analysis of PBMC cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).

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