ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d,mitochondrial antibody|ATP synthase,H+ transporting,mitochondrial F0 complex,subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody
|产品名称||Anti-ATP5PD Antibody(monoclonal, 6B12)|
|免疫原||E.coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H,respectively.|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis,utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core,F1,and the membrane-spanning component,Fo,which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha,beta,gamma,delta,and epsilon) assembled in a ratio of 3 alpha,3 beta,and a single representative of the other 3. The Fo seems to have nine subunits (a,b,c,d,e,f,g,F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition,three pseudogenes are located on chromosomes 9,12 and 15.|
|Uniprot ID||ATP5H: O75947|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Immunohistochemistry in paraffin section:||1:50-400|
|Immunocytochemistry in fixed cells:||1:50-400|
|(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.|
Figure 1. Western blot analysis of anti-ATP5H antibody (M09565). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela tissue lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human U-87MG whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: monkey COS-7 whole cell lysates,Lane 6: human SW620 whole cell lysates.Lane 7: rat PC-12 whole cell lysates.Lane 8: mouse Ana-1 whole cell lysates.Use rabbit anti- ATP5H 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP5H at approximately 22KD. The expected band size for ATP5H is at 18KD.
Figure 2. IHC analysis of anti- ATP5H antibody (M09565).detected in paraffin-embedded section of human B lymphocytic tumorT tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of anti- ATP5H antibody (M09565).detected in paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. ICC analysis of anti- ATP5H antibody (M09565).was detected in immunocytochemical section of A549 cells. Cells were stained using the Dylight cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)Catalog # BA1032) and counterstained with DAPI (blue).