Putative Polycomb group protein ASXL1|Additional sex combs-like protein 1|ASXL1|KIAA0978
|产品名称||Anti-ASXL1 Antibody(monoclonal, 11I4)|
|免疫原||A synthetic peptide corresponding to a sequence of human ASXL1 (KKERTWAEA ARLVLENYSDAPMTPKQILQVIEAE).|
|内容||500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA|
|纯化方式||protein G purified.|
|保存条件||12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.|
|背景资料||Putative Polycomb group protein ASXL1 is a protein that in humans is encoded by the ASXL1 gene. This gene is similar to the Drosophila additional sex combs gene,which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins,which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas,enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants.|
|Uniprot ID||ASXL1: Q8IXJ9|
|推荐配套的二抗和检测试剂||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.|
|Flow Cytometry:||1-3μg/1x106 cells|
Figure 1. Western blot analysis of anti- ASXL1 antibody (M01099).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human COLO-320 tissue lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Jurkat whole cell lysates,Lane 5: human HepG2 whole cell lysates,Lane 6: human Caco-2 whole cell lysates.Lane 7: rat PC-12 whole cell lysates.Lane 8: mouse RAW246.7 whole cell lysates.Use rabbit anti- ASXL1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ASXL1 at approximately 165KD. The expected band size for ASXL1 is at 165KD.
Figure 2.Flow cytometry analysis of Caco-2 cell HELA(1x106) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody.Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).