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Anti-ALIX/PDCD6IP Antibody(monoclonal, 14D10)

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一抗 - 单克隆抗体

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Figure 1. Western blot analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates<br>Lane 2: human HepG2 whole cell lysates<br>Lane 3: human Jurkat whole cell lysates<br>Lane 4: human PANC-1 whole cell lysates<br>Lane 5: human K562 whole cell lysates<br>Lane 6: human SW579 whole cell lysates<br>Lane 7: rat RH35 whole cell lysates<br>Lane 8: mouse NIH3T3 whole cell lysates<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDCD6IP antigen affinity purified monoclonal antibody (Catalog # M01751-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDCD6IP at approximately 96-100KD. The expected band size for PDCD6IP is at 96KD. Figure 2. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 3. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 4. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 5. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 6. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 7. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. Figure 8. IF analysis of PDCD6IP using anti- PDCD6IP antibody (M01751-1). <br>PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Figure 9. Flow Cytometry analysis of A431 cells using anti- PDCD6IPantibody (M01751-1).<br>Overlay histogram showing A431 cells stained with M01751-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PDCD6IP Antibody (M01751-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
  • Figure 1. Western blot analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates<br>Lane 2: human HepG2 whole cell lysates<br>Lane 3: human Jurkat whole cell lysates<br>Lane 4: human PANC-1 whole cell lysates<br>Lane 5: human K562 whole cell lysates<br>Lane 6: human SW579 whole cell lysates<br>Lane 7: rat RH35 whole cell lysates<br>Lane 8: mouse NIH3T3 whole cell lysates<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDCD6IP antigen affinity purified monoclonal antibody (Catalog # M01751-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDCD6IP at approximately 96-100KD. The expected band size for PDCD6IP is at 96KD.
  • Figure 2. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
  • Figure 3. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
  • Figure 4. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
  • Figure 5. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
  • Figure 6. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
  • Figure 7. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
  • Figure 8. IF analysis of PDCD6IP using anti- PDCD6IP antibody (M01751-1). <br>PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
  • Figure 9. Flow Cytometry analysis of A431 cells using anti- PDCD6IPantibody (M01751-1).<br>Overlay histogram showing A431 cells stained with M01751-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PDCD6IP Antibody (M01751-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

M01751-1

860元/50ul  1520元/100ul  2280元/150ul  

human,mouse,rat

WB,IHC-P,ICC/IF,FCM

Monoclonal

现货

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Product Brief

  • 产品概况

    货号 M01751-1
    产品名称 Anti-ALIX/PDCD6IP Antibody(monoclonal, 14D10)
    基因名 PDCD6IP
    抗体来源 Mouse
    克隆 Monoclonal
    抗体亚型 Mouse IgG2b
    分子量 96KD
    免疫原 E.coli-derived human PDCD6IP recombinant protein (Position: A2-D330). Human PDCD6IP shares?96.7% and 95.2% amino?acid?(aa)?sequence?identity?with mouse?and rat PDCD6IP, respectively.
    内容 Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
    纯化方式 Immunogen affinity purified.
    浓度 200ug/ml
    产品形态 Lyophilized
    保存条件 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
    Uniprot ID PDCD6IP: Q8WUM4
    推荐配套的二抗和检测试剂 Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-146) for IHC(P). *Blocking peptide 可以联系我们购买。

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    Figure 1. Western blot analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates<br>Lane 2: human HepG2 whole cell lysates<br>Lane 3: human Jurkat whole cell lysates<br>Lane 4: human PANC-1 whole cell lysates<br>Lane 5: human K562 whole cell lysates<br>Lane 6: human SW579 whole cell lysates<br>Lane 7: rat RH35 whole cell lysates<br>Lane 8: mouse NIH3T3 whole cell lysates<br>After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDCD6IP antigen affinity purified monoclonal antibody (Catalog # M01751-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDCD6IP at approximately 96-100KD. The expected band size for PDCD6IP is at 96KD.

    Figure 1. Western blot analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).
    Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: human Hela whole cell lysates
    Lane 2: human HepG2 whole cell lysates
    Lane 3: human Jurkat whole cell lysates
    Lane 4: human PANC-1 whole cell lysates
    Lane 5: human K562 whole cell lysates
    Lane 6: human SW579 whole cell lysates
    Lane 7: rat RH35 whole cell lysates
    Lane 8: mouse NIH3T3 whole cell lysates
    After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PDCD6IP antigen affinity purified monoclonal antibody (Catalog # M01751-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PDCD6IP at approximately 96-100KD. The expected band size for PDCD6IP is at 96KD.

    Figure 2. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 2. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).
    PDCD6IP was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 3. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 3. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).
    PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 4. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 4. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).
    PDCD6IP was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 5. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 5. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).
    PDCD6IP was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 6. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 6. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).
    PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 7. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).<br>PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 7. IHC analysis of PDCD6IP using anti-PDCD6IP antibody (M01751-1).
    PDCD6IP was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PDCD6IP Antibody (M01751-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

    Figure 8. IF analysis of PDCD6IP using anti- PDCD6IP antibody (M01751-1). <br>PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    Figure 8. IF analysis of PDCD6IP using anti- PDCD6IP antibody (M01751-1).
    PDCD6IP was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti- PDCD6IP Antibody (M01751-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    Figure 9. Flow Cytometry analysis of A431 cells using anti- PDCD6IPantibody (M01751-1).<br>Overlay histogram showing A431 cells stained with M01751-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PDCD6IP Antibody (M01751-1, 1μg/1x10<sup>6</sup> cells) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10<sup>6</sup> cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10<sup>6</sup>) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

    Figure 9. Flow Cytometry analysis of A431 cells using anti- PDCD6IPantibody (M01751-1).
    Overlay histogram showing A431 cells stained with M01751-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PDCD6IP Antibody (M01751-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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